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Ensure that the information you fill in is correct. Once the verification is successful, you can proceed with the normal download. Determination and monitoring of Catherin-B cell activity over time in vitro. The magic red substrate in this assay is colored red by cleavage of active catherin enzymes. Analyze the fluorescence signal using fluorescence microscopy or a fluorescence plate reader.
Elevated β-catenin enzyme activity in serum or extracellular matrix may indicate several pathological conditions. Researchers use ICT’s Magic Red assay kits to evaluate and monitor β-catenin activity in cultured cells and tissues. This screening kit detects the activity of Katherine B. To use Magic Red, add the substrate directly to the cell culture media, incubate, and screen. Because it is cellular permeable, it readily penetrates the cell membrane and membranes of intracellular organelles—no lysis or permeabilization steps required. If the cathepsin enzymes are active, the magic red substrate is bound and upon excitation the cresyl will fluoresce violet. When enzyme activity and a substrate are coated in magic red, the signal will be amplified, allowing researchers to monitor the evolution of color over time. Samples can be analyzed by fluorescence microscopy or a fluorescent plate reader. Hoechst 33342 and Acridine Orange are included in the kit to detect nuclear morphology and lysosomal organelle structure, respectively.
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Rato, E; Chowdhury, SR; Sv, N.S.; Schneider, M; Whitman, M; Helm, D; Palm, W. Direct control of lysosomal catabolic activity by mTORC1 through regulation of V-ATPase recruitment. Nature Communications. August 17, 2022; doi: 10.1038/s41467-022-32515-6. condition
Yes Yes ; Kakuda, K.; Imochimura, C; Hara, N.; Hasegawa, J; Terawaki, S; Kimura, Y; Ikenaka, K.; Suetsugu, S; Mochizuki, H; Imoshimori, T; Nakamura, S. PACSIN1; It is required for amphisome-lysosome fusion during basal autophagy and components of selective autophagy. PLoS Genetics. June 1, 2022; doi: 10.1371/.journal.pgen.1010264. text
Hmm; fiber. Wang, C; Feng, X; Jing, Q; Chen, W.; Marty, M; Chang, W.; Gao, C.; Reed, W.; Swanson, Y.; Du, W.; hmm; RI; Xu, H. Parkinson risk protein for Parkinson’s disease TMEM175 is a proton-activated proton channel in lysosomes. cell. June 23, 2022; doi: 10.1016/j.cell.2022.05.021. full text
Tanno, T.; Chang, F.; Bailey, C.; Wang, Y; Itteprasert, W.; Davenport, M; Cheng, F.; Liu, Y. An aptamer-based small RNA delivery platform and its application in cancer therapy. Genes and diseases. May 1, 2022; doi: 10.1016/j.gendis.2022.05.004. full text
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Chung, W.; Rao, Z; a little. sun, p.; huh; Wang, P; chia, y; pan, X; Tang, W.; qin, zi; Zhou, h. Wang, X. Defective vasculature promotes cellular extravasation of mitochondrial DNA to activate STING signaling during sterile liver infection in senescent macrophages. Old dungeon. May 22, 2022; doi: 10.1111/jcpe.13647. a summary
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